Association of adiponectin gene rs2241766 polymorphism with serum adiponectin level, Insulin resistance and type 2 diabetes mellitus in an Iranian population

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Introdution: Adiponectin is a peptide secreted from fat cells that plays an important role in the development of insulin resistance and type 2 diabetes. A number of single nucleotide polymorphisms in the adiponectin gene are associated with lower adiponectin expression and T2DM. The aim of this study was to determination the frequency of rs 2241766 polymorphism in adiponectin gene and its relation with adiponectin level, insulin resistance, type 2 diabetes and in pre diabetes/diabetes and healthy individuals. Methods: This study was performed in a case -control method. 73 prediabetic/diabetic patients with fasting blood sugar >100 mg/dl was selected as the case group without drug history, and 76 individuals with FBS 70-100 mg/dl as the control group. Biochemical and anthropometric variables were measured by standard methods and the levels of adiponectin and insulin hormone were measured using commercially available human ELISA kit. The determination of genotyping was done using PCR-RFLP method. Data were analyzed by SPSS software version 19. Results: Regression analysis showed that polymorphism rs2241766 in the adiponectin gene has no significant relation with diabetes and insulin resistance. No significant difference was found between TT and TG carriers for any of the variables in the two groups (pre diabetes/diabetes and Non-diabetic). A negative correlation was seen between adiponectin and insulin resistance ( r=-0.225, p< 0/01). Conclusion: According to this study, adiponectin gene polymorphism rs2241766 is not associated with adiponectin levels, insulin resistance and type 2diabetes, but there was a correlation between adiponectin and insulin resistance.

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عنوان ژورنال

دوره 27  شماره 2

صفحات  1216- 1227

تاریخ انتشار 2019-05

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